Author(s):
1. Jelena Živković, University of Niš Faculty of Medicine, Serbia
2. Milena Radenković, University of Niš Faculty of Medicine, Serbia
3. Sanja Stojanović, University of Niš Faculty of Medicine, Serbia
4. Jelena Najdanović, University of Niš Faculty of Medicine, Serbia
5. Vladimir Cvetković, University of Nis, Faculty of Sciences and Mathematics, ::Visegradska 33, Serbia
6. Marija Vukelić-Nikolić, Institut za biologiju i humanu genetiku, Medicinski fakultet, Univerzitet u Nišu, 1800 Niš, Srbija, Serbia
7. Ivica Vučković, Clinic for dental medicine, Niš, Serbia
8. Nenad Ignjatović, Institute of Technical Sciences of the Serbian Academy of Sciences and Arts, Belgrade, Serbia, Serbia
9. Stevo Najman, Faculty of Medicine, University of Niš, Serbia

Abstract:
Today's approach to tissue repair and regeneration often involves application of different biomaterials, alone or in combination with cells and/or growth factors. All materials, regardless of whether they are of natural origin or synthesized, cause a reaction in the surrounding tissue after implantation. This reaction involves a series of related events on which ultimately depends whether the implanted material will be well accepted and perform its primary role. Each material represents a foreign body for the organism and initiates a transient inflammatory reaction, the duration and intensity of which largely determine materil's further fate. Inflammatory reaction is influenced by material's chemical composition, the size and shape of its granules, porosity, compactness, as well as the degree of material's biodegradability. Thus, the fate of the material can go one of two ways: towards good integration with the surrounding tissue, supporting the cells with which it interacts and, in the case of biodegradable materials, replacement by healthy, functional tissue, or it will cause a strong inflammatory response resulting in the complete isolation of the material from the rest of the tissue with thick fibrous capsule. In vivo research of tissue response to implanted biomaterial involves investigation in different animal models and in different tissues, with orthotopic or ectopic implantations. We used orthotopic models for bone formation in rats and rabbits and models of subcutaneous implantation of various biomaterials in mice and rats. Numerous methods were used for the analysis of implants and surrounding tissue in the time periods of early and late response, such as histological staining, histomorphometry, immunohistochemistry, SEM, radiographic methods, analysis of specific gene expression and others. In vivo animal models for the purpose of preclinical studies are important in order to obtain guidelines for clinical application.

Key words:
material,tissue repair,tissue regeneration,implantation,in vivo

Thematic field:
SYMPOSIUM B - Biomaterials and nanomedicine

Date of abstract submission:
16.08.2022.

Conference:
Contemporary Materials 2022 - Savremeni materijali

Abstract